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Techniques

Behavioral Monitoring of Motivated Behaviors

Innate approach/avoidance behaviors

Anxiety-related
behaviors following stress

Drug self-administration
and preference

In-vivo and ex-vivo single-cell calcium imaging

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In-vivo GCaMP6F activity monitored via a GRIN lens

Ex-vivo GCaMP6F

In-vivo fiber photometry: imaging bulk calcium and biosensors

Calcium activity in response
to aversive stimuli

Acetylcholine release in response
to an aversive odor

Baseline

Stimulus

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Neural circuit manipulations: chemo- and optogenetics

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High-resolution Microscopy

3D morphological reconstruction of a VP cholinergic neuron

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3D morphological reconstruction of a nucleus accumbens astrocyte

Neuroanatomical tracing

Anterograde tracing of cholinergic projections

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Retrograde mapping of BLA-projecting cholinergic neurons

Activity mapping of engaged neurons

Whole brain slice imaging of cFos+ cholinergic neurons

Immunohistochemistry & RNAscope

IHC for GFAP

IHC for markers of distinct BLA neurons

RNAscope to assay mRNA expression in the VP

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